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KMID : 1143120130030010050
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Asia Pacific Allergy
2013 Volume.3 No. 1 p.50 ~ p.58
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Murine subcutaneous immunotherapy models with beneficial immunological and physiological effects
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Chang Yoon-Seok
Kim Yoon-Keun
Kim Sae-Hoon
Park Heung-Woo
Min Kyung-Up
Kim You-Young
Cho Sang-Heon
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Abstract
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Background: Immunotherapy was introduced 100 years ago and has a unique role in the treatment of allergic diseases in that only immunotherapy can induce long-term immunological tolerance. However, only a few mouse models of immunotherapy have been developed so far.
Objective: We tried to establish murine immunotherapy models that have similar findings in human using subcutaneous rush immunotherapy-like schedule.
Methods: To determine the maximal safe or maximal tolerable dose, injection dose was doubled twice a day from the dose of sensitization. Mice with established asthma using ovalbumin (OVA) were repeatedly injected with OVA from the dose of sensitization subcutaneously twice a day: after reaching to the maximal safe or maximal tolerable dose, mice were injected with each dose either 10 times or 24 times.
Results: Short term immunotherapy (10 times) with the maximal safe and tolerable dose of OVA showed decreased IL-5 production, decreased IL-5/INF-γ ratio, and increased IgG2a/IgG1 but there was no significant difference in airway hyperresponsiveness (AHR) or airway inflammation. Prolonged immunotherapy (24 times) with the maximal tolerable dose not only decreased cytokine productions of IL-5 and even INF-γ, but also decreased IgE, IgG1 and even IgG2a production. Remarkably, the prolonged immunotherapy provided a protective effect on AHR.
Conclusion: This study suggested immunotherapy models with some beneficial immunological and physiological effects in murine asthma.
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KEYWORD
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Asthma, Animal models, Allergy, Immunotherapy
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